This is a crowdsource page for people who want to post their findings on their attempts to validate the STAP stem cell ( ＳＴＡＰ細胞) method. I’m going to color successful or even moderately encouraging reports green and failures/discouraging results in red
red 9 green 1
http://tokyocicada.blog.fc2.com/blog-entry-59.html
http://www.ipscell.com/stap-new-data/
Felix wrote on 3/24/14
We have been repeating the experiment with MEFs from OG2 mice. We indeed observed a significant increase of GFP positive population 4 days after acid (pH5.7) treatment, however almost all GFP positive cells are also showing strong red/blue fluorescence under the microscope, indicating the presence of intense cellular autofluorescence after treatment.
Ray and Sandy wrote on 2/19/14:
We used MEF and treated in HBSS at normal pH or titrated to pH5.7 with HCl as described in the manuscript in suspension culture (~5×10^5 cells in 2mL DMEM/F12 media + 2% B27 + LIF). On day 1, some of the MEF formed small clumps in suspension, but this happened with and without low pH treatment. On day 7, the cells were plated down onto slides using cytospin, fixed/permeabilized and stained for Oct4. Cells in all conditions appeared Oct4 negative (results attached). Please note that the culture medium was not changed during the 7 day incubation period as it was not mentioned in the manuscript.
